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Wednesday, October 22, 2014

IBA's Cost-effective and easy to handle biotin removal

Biotin is often present in cell culture media. Its nearly irreversible binding to Strep-Tactin has to be taken into account for two reasons when recombinant proteins secreted to the cell culture supernatant are aimed to be directly purified via Strep-Tactin affinity chromatography:
  • It efficiently competes with the (Twin-)Strep-tag target proteinthereby preventing it from getting bound to the resin
  • it inactivates Strep-Tactin resins and thus make a re-use impossible.
BioLock is a ready-to-use and cost effective solution for the removal of free biotin from cell culture supernatant. It simply has to be added to the cell culture supernatant prior to purification.
The required amount of BioLock for effective biotin blocking can easily be determined since 1ml BioLock blocks 70 μg biotin. In addition we provide a table with the required BioLock amounts for standard mammalian and insect cell culture media. More information (pdf 1.1MB)
For detailed product information please download the data sheet.

For additional information contact info@stratech.co.uk.

Product Focus: Shrimp Alkaline Phosphatase + Exonuclease I for cleanup of PCR products

Jena Bioscience's SAP-Exo Kit is a quick, easy and reliable enzymatic cleanup reagent to remove excess primers and dNTPs within 15 minutes. The kit is specially recommended to clean-up PCR products for subsequent applications like sequencing, genotyping, cloning or SNP analysis.
  • Fast 15 minute protocol
  • No loss of DNA - 100% recovery rate
  • Complete removal of primers and nucleotides
  • Fully scalable to different reaction sizes
  • No interference with downstream applications
  • Easy to automate

SAP-Exo Kit

Shrimp Alkaline Phosphatase + Exonuclease I for cleanup of PCR products
Product Cat. No. Amount
S pack PP-218S 200 reactions
L pack PP-218L 1000 reactions

Datasheet for SAP-Exo Kits

SAP-Exo Kit is an enzymatic cleanup reagent for quick, easy and reliable PCR purification

Product Focus: dNTPs & dNTP Pre-Mixes

Premium Quality dNTPs – at prices you can’t ignore…!

The synthesis of a complementary DNA/cDNA strand by enzymatic polymerization of deoxynucleotide triphosphates (dNTPs) is the basic principle of DNA amplification, sequencing and cDNA synthesis techniques such as PCR, real-time PCR and reverse transcription. For these, amplification sensitivity, product yield and reproducibility are strongly dependent on the quality of the reagents used.
Jena Bioscience’s enzymatic dNTP manufacturing process ensures superior quality for deoxynucleotide triphosphates: All their dNTPs are ultrapure (> 99 % by HPLC) and functionally tested by a set very sensitive enzymatic reactions (see below dNTP Guide and dNTP specifications).



 

dNTP Mix / 10 mM

Premix of 10 mM dATP, dCTP, dGTP and dTTP


Product Cat. No. Amount
S pack NU-1006S-JEN 200 µl
L pack NU-1006L-JEN 1 ml

 

dNTP Mix / 25 mM

Premix of 25 mM dATP, dCTP, dGTP and dTTP


Product Cat. No. Amount
S pack NU-1023S-JEN 200 µl
L pack NU-1023L-JEN 1 ml

 

dNTP Mix incl. dUTP / 10 mM

Premix of 10 mM dATP, dCTP, dGTP and 20 mM dUTP


Product Cat. No. Amount
S pack NU-1020S-JEN 200 µl
L pack NU-1020L-JEN 1 ml

 

dNTP Mix GCamplifier / 10 mM

Modified dNTP Mix for amplification of GC-rich sequences


Product Cat. No. Amount
Kit PCR-257-JEN 100 µl

dNTP PCR Mix GCamplifier is specially designed for amplification of GC-rich DNA templates. The kit contains a modified dGTP analog that significantly reduces the stability of GC-rich sequences thereby enhancing PCR efficiency. The dNTP PCR Mix GCamplifier is used instead of a standard dNTP mix.



Where can I find more information about Jena Bioscience?
Visit the manufacturer page at www.stratech.co.uk/jena_bioscience, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.