Tuesday, May 19, 2015

Supplier Focus: System Biscience – Stem Cell Research, Tools to Study Pluripotency and Differentiation

iPSC Technologies and Reporting Systems

  • Induce Pluripotency with iPSC factors
  • Study Pluripotency with pre-made iPS Cell lines
  • Confirm Pluripotency with Oct4 and Nanog
  • Track Differentiation across 5 lineages


 

iPSC Reprogramming Systems


Episomal
A non-viral, non-integrating vector system which enables high efficiency reprogramming of many somatic cell types into iPSCs. This system offers one of the most effective options for generating transgene-free iPSCs, and is easy to use.
Make Transgene-free iPSCs
  • Complete mixture with OSK, L-Myc
    shRNA-p53, miR302/367 and GFP
  • Simple protocol for reliable reprogramming
  • Reprogram elderly fibroblast cells efficiently
  • Compatible with feeder or feeder-free conditions

Integrase
A non-viral system utilizing the PhiC31 integrase, especially effective for generation of murine iPSCs. Popular for gene therapy applications, the single copy, integrated cassette includes a unique PinPoint site which can be used for targeted gene addition.
Make nonviral iPSCs
  • All-in-one iPSC vector
  • Single copy integration
  • Removable with FLP
  • Highly efficient

PiggyBac
A non-viral method based on the piggyBac transposon system. The piggyBac transposase can be used to excise the iPSC factors after reprogramming.
Cut and Paste Transposition
  • One transfection makes transgenic cell lines
  • Effective in Human, Mouse and Rat cells
  • No cargo limit — integrate 10-100kb
  • All-in-one tight inducible PiggyBac vector
  • PiggyBac copy number qPCR kit

Retroviral (MicroRNA Reprogramming Lentivirus Guide, Minicircle iPSC Technology User Manual, Guide to Viral iPSC Reprogramming)
The original method for generating iPSCs. A well-established, high efficiency system for creating iPSCs in any lab.
Study pluripotency
  • Oct4
  • Nanog and Sox2
  • KLF4 or Lin28
  • c-Myc



Where can I find more information about SBI?

Visit the manufacturer page at www.stratech.co.uk/SBI, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.

Product Focus: Microplate Based Snapshot ChIP Assay

Different gene expression depends not only on the binding of specific transcription factors (TFs) to discrete promoter elements but also on expression and interaction of co-activators with its associated TFs. The interactive cofactor complexes allow the functional integration of multiple TFs and pathways facilitating the formation of distinct biological programs. Unlike electrophoretic mobility shift assays detecting the binding of specific TFs present in cell lysates to DNA sequences in vitro, chromatin immunoprecipitation (ChIP) assay is enable the analysis of the association of a specific TF or co-factor with specific promoters in vivo and provides a snapshot of how a regulatory TF or co-factor affects the expression of a single gene or a variety of genes at the same time.
To simplify the ChIP assay in a high throughput method, Signosis has developed a 96-well microplate-based ChIP assay, where all of steps from IP to DNA elution are handled in microplate wells without sample transfers in an high throughput way. The assay is an ideal tool for studying up to 96 protein-DNA bindings in one sample or one protein-DNA binding in up to 96 samples, or multiple protein-DNA bindings in multiple samples/treatments.

Benefits:
  • High Throughput – 96 ChIP experiments can be performed simultaneously in one microplate.
  • Simple Sample Handling – All steps from IP to DNA elution are done in a single well, including crosslinking and fragmentation, without the need to transfer samples.
  • Reproducible and Time Saving – Plate-based ChIP assay eliminates errors introduced from conventional bead-based individual precipitation, washing and elution steps.
  • Affordable – More economical per reaction than conventional ChIP assay.


Principle

ChIP assays reveal complexity for chromatin, transcriptional regulation, RNA processing and DNA replication. These processes are regulated by multiple histone modifications that can be mediated by kinases, methyltransferases, acetyltransferase, ubiquitintransferase and others. In addition, hundreds of different RNA processing and transcription factors are involved in these epigenetic changes. As a result, the regulatory modifications of DNA-bound and accessory factors may be far greater in number and more complex than previously reported. Therefore, the ability to simultaneously detect multiple modification events will enhance our understanding of nuclear processes. The conventional ChIP method has notable limitations, including time-consuming procedure, bead-based individual IP and multiple tube transfers. These limitations result in potential sample loss, cross contamination and variability. It becomes a bigger problem when multiple chromatin samples are involved. Signosis provides a microplate-based chromatin ChIP assay kit, which allows monitoring the nuclear events in a high throughput way. The kit is optimized to perform all of IP steps from capturing the protein/DNA complexes to eluting DNA in one single well.


 


 User Manuals and Product Information

Microplate Snapshot ChIP Assay CP-0001

Oct4 Microplate Snapshot ChIP Assay CP-0101
NRF2 Microplate Snapshot ChIP Assay CP-0102

HDAC1 Microplate Snapshot ChIP Assay CP-0103
Smad Microplate Snapshot ChIP Assay CP-0104

Stat3 Microplate Snapshot ChIP Assay CP-0105
NFkB Microplate Snapshot ChIP Assay CP-0106
HIF Microplate Snapshot ChIP Assay CP-0107
H3 Microplate Snapshot ChIP Assay CP-0108
p300 Microplate Snapshot ChIP Assay CP-0109
p53 Microplate Snapshot ChIP Assay CP-0110


Where can I find more information about Signosis?

Visit the manufacturer page at www.stratech.co.uk/signosis, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.


Monday, May 18, 2015

Product Focus: aCella™ – SEAP: Secreted Alkaline Phosphatase (SEAP) Chemiluminescent Reporter Gene Assay Kit

Secreted alkaline phosphatase (SEAP) is widely used as a reporter in the study of gene expression. SEAP is secreted into the culture supernatant of the transfected cell lines,  its activity in the culture medium reflects changes in intracellular concentrations of SEAP mRNA and protein. The changes of gene expression can be easily assayed using repeated sampling of cell cultures.

Cell Technology’s aCella – SEAP detection kit utilises a Chemiluminescent-based assay technique to detect SEAP reporter gene expression. It provides an extremely sensitive tool to quantitate SEAP and analyse transcriptional and promoter activity in transfected cells.

Key Benefits:

  • Highly effective and stable chemiluminescent assay for SEAP.
  • Extremely sensitive, detect alkaline phosphatase at attogram level.
  • Simple and fast assay-add the reagent directly to your experimental samples. Plate can be incubated and read in 5 min.
  • Works for cell culture media and tissue lysates.



Where can I find more information about Cell Technology?

Visit the manufacturer page at www.stratech.co.uk/cell_technology, email info@stratech.co.uk or call  +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.