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Monday, March 16, 2015

Bovine Leukemia Inhibitory Factor (LIF) Recombinant Protein

Kingfisher Biotech’s Bovine Leukemia Inhibitory Factor (LIF) recombinant protein can be used in cell culture, as an LIF ELISA Standard, and as a Western Blot Control. Leukemia inhibitory factor, or LIF, is a member of the IL-6 family. LIF affects cell growth by inhibiting differentiation. When LIF levels drop, the cells differentiate. Leukemia inhibitory factor derives its name from its ability to induce the terminal differentiation of myeloid leukemic cells, thus preventing their continued growth. Other properties attributed to LIF include: the growth promotion and cell differentiation of different types of target cells, influence on bone metabolism, cachexia, neural development, embryogenesis and inflammation. p53 regulated LIF has been shown to facilitate implantation in the mouse model and possibly in humans. Removal of LIF pushes stem cells toward differentiation, but they retain their proliferative potential or pluripotency. LIF is typically added to stem cell culture medium to reduce spontaneous differentiation.Kingfisher Biotech’s Bovine Leukemia Inhibitory Factor (LIF) recombinant protein is intended for use in animal model and animal health research.


Product Catalogue No. Quantity
Bovine Leukemia Inhibitory Factor (LIF) Recombinant Protein – 100 micrograms RP0997B-100 100 ug
Bovine Leukemia Inhibitory Factor (LIF) Recombinant Protein – 25 micrograms RP0997B-025 25 ug
Bovine Leukemia Inhibitory Factor (LIF) Recombinant Protein – 5 micrograms RP0997B-005 5 ug


Where can I find more information about Kingfisher Biotech?

Visit the manufacturer page at www.stratech.co.uk/kingfisher-biotech-inc, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.

NEW Products: aCella™ – TOX: Bioluminescence Non Radioactive Cytotoxicity Assay (GAPDH)

Key Benefits

  • Safe – Non radioactive enzyme release assay
  • Versatile – Assay can be run in serum-supplemented media
  • Homogenous – One-step, no wash assay which can be run in same plate as samples
  • FAST – Results in 3-5 minutes
  • Highly Sensitive – can detect less than 500 cells/well in 10% FCS
  • Adaptable for High Throughput screening
  • Non-destructive assay allows monitoring of additional parameters.
  • Perfect for ADCC, CMC & Complement Cytotoxicity assays

Description

Cell Technology offers aCella-TOX, a highly sensitive assay using its patented coupled luminescent technology for the detection of cytotoxicity. This assay is designed to quantitatively measure the release of Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) from mammalian cell lines or bacterial cells (1,2,3,4). Other enzyme release assays suffer from low sensitivity as a result of interference by serum or phenol red present in the media (i.e. Lactate Dehydrogenase (LDH release assay (5,6,7,8)).

The release of GAPDH is coupled with the enzyme 3-Phosphoglyeric Phosphokinase (PGK) to produce ATP. ATP is detected with aCella-TOX as a homogeneous assay that can measure cytoxicity or cell viability via the Luciferase-Luciferin Bioluminescence methodology. It is also possible to set up kinetic assays by transferring culture supernatants from the original assay plate onto a different plate. Additional parameters such as gene expression can also be monitored as the assay system is non-destructive.






Fig A: A Direct comparison of 51Cr and aCella-TOX was carried out with the same donors with Daudi Cells. Log (EC50) value for aCella-Tox was -2.23, and that for 51Cr was -2.087 for Donor A.


 

Fig B: A Direct comparison of 51Cr and aCella-TOX was carried out with the same donors with Daudi Cells. Log (EC50) value for aCella-TOX was -2.612, and that for 51Cr was -2.77 for Donor B.



Where can I find more information about Cell Technology?

Visit the manufacturer page at www.stratech.co.uk/cell_technology, email info@stratech.co.uk or call  +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.

NEW: hAstroPro Human Astrocyte+Astroglial-Neurons Co-Culturing Kits

Neuromics now provides ArunA Biomedical’s  first of  their kind human astrocyte-glial or astrocyte-glial-neuron co-culturing kits to meet your exacting specifications. Whether you require a pure astro-glial or mixed culture, we are proud to be the first offering you the right solution. Options:

  1. hAstroPro™ Human Astrocyte Cells are derived from human pluripotent stem cell line h9 (NIH registry number 0062) and are the first highly characterized hESC derived CNS support cell type commercially available. hAstroPro™ cells are derived using a chemically defined method that enables shortened differentiation and scaled uniform production.
  2. hAstroPro™+NeuroNet™ Pure Human Neurons are a highly pure population of hPSC derived neurons developed with versatility in mind. As a standalone model for studies of cellular and molecular neurobiology, drug discovery and neurotoxicity, NeuroNet™ Human Neurons enable rapid response in a relevant in vitro culture model system and have been strategically designed for co-planer cellular model studies.



Images: Astrocyte-Glial-Neuron Co-Culture Stained with MAP-2 (Green) and DAPI (Blue).


hAstroPro Human Astrocyte Kit

Cell Type:  Astrocytes
Part Number:  hAP7027
Species:  human
Source:  derived from h9 hESC (NIH registry number 0062)
Morphology:  glial
Quantity:  ~1×106cells per vial
Shipping method:  dry ice
Storage:  liquid nitrogen
Growth Properties:  adherent
Medium:  AB2™ Basal Neural Medium with ANS™ Neural Medium Supplement plus additional unsupplied reagents.
Purity as determinded by qPCR: S100β, CD44, BDNF, GFAP.


NeuroNet Pure Human Neurons

Quantity:  ~1×106 cells per vial
Shipping method:  dry ice
Storage:  liquid nitrogen
Growth Properties:  adherent, non-proliferative
Medium:  AB2™ Basal Neural Medium with ANS™ Neural Medium Supplement plus LIF (10 mg/mL)
Phenotyping:  Cells are >90% β III tubulin positive and >60% MAP2 positive.
DNA fingerprint:  Results are consistent with the presence of a single cell line with no evidence of culture cross contamination. The loci match the DNA fingerprint pattern for the H9 (NIH designation, WA09) hESC line as published in http://stemcells.nih.gov/research/nihresearch/scunit/.
Pathogen testing:   This lot was derived from the H9 hESC line that has been tested for Hepatitis B, Hepatitis C, HIV-1, HIV-2, HTLV-I/II, HSV1, HSV2, EBV, and CMV.  The H9 cell line has been tested and shown to be negative (performed by GIVF Laboratories).  Cells are tested for sterility and mycoplasma contamination.



Where can I find more information about Neuromics?

Visit the manufacturer page at www.stratech.co.uk/neuromics, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe.

Please contact us to find out which product ranges we can supply in your country.