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Friday, April 4, 2014

Product Focus: PowerFluor™ Immunofluorescence Mouse Detection kit

PowerFluor™immunofluorescence mouse detection kit is designed to localize mouse primary antibodies on human, goat and rabbit tissue in immunofluorescence applications. PowerFluor™ uses biotin-free technology and it completely eliminates the potential background due to endogenous biotin activity on biotin-rich tissues when use Biotin-Strepavidin-Fluorphore technology. The MaxFluor™ Dyes range exhibit bright fluorescence intensity and photostability. Also they remains highly fluorescent over a broad pH range (pH 4-9). This make them an ideal choice for researcher’s immunofluorescence applications. PowerFluor™ uses a proprietary fluorescence signal amplification technology so the researchers may expect the best signal-to-noise ratio using the PowerFluor™ImmunoFluorescence Detection Kit when compare with the direct labeling technology or other indirect methods, such as Biotin-Streptavidin method.

Key Benefits:
  • Bright and stable fluorescence
  • Biotin-free signal amplification
  • Super clean background
  • Significant increase in fluorescence signal
  • 3-4 times more sensitive and cleaner background than direct method
  • No biotin blocking steps and completely elimination of biotin background
  • Best signal-to-noise ratio


 

For immunofluorescence detecting mouse primary antibodies on human, rabbit and goat specimen

 

Cat No. Description Size
Dye
Primary Antibody
Tissue
Datasheet
PF11-M-MAX PowerFluor™488 Immunofluorescence Mouse Detection Kit
18ml
MaxFluor 488
Mouse
Hu, Rb, Gt
PF11-S-MAX PowerFluor™488 Immunofluorescence Mouse Detection Kit
6ml
MaxFluor 488
Mouse
Hu, Rb, Gt
PF12-M-MAX PowerFluor™550 Immunofluorescence Mouse Detection Kit
18ml
MaxFluor 550
Mouse
Hu, Rb, Gt
PF12-S-MAX PowerFluor™550 Immunofluorescence Mouse Detection Kit
6ml
MaxFluor 550
Mouse
Hu, Rb, Gt
PF13-M-MAX PowerFluor™594 Immunofluorescence Mouse Detection Kit
18ml
MaxFluor 594
Mouse
Hu, Rb, Gt
PF13-S-MAX PowerFluor™594 Immunofluorescence Mouse Detection Kit
6ml
MaxFluor 594
Mouse
Hu, Rb, Gt
PF14-M-MAX PowerFluor™650 Immunofluorescence Mouse Detection Kit
18ml
MaxFluor 650
Mouse
Hu, Rb, Gt
PF14-S-MAX PowerFluor™650 Immunofluorescence Mouse Detection Kit
6ml
MaxFluor 650
Mouse
Hu, Rb, Gt


Where can I find more information about MaxVision?

Visit the manufacturer page at www.stratech.co.uk/maxvision, email info@stratech.co.uk or call  +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.

Product Focus: VeriQuest Probe qPCR Master Mix

VeriQuest Probe qPCR Master Mix is supplied as a 2X pre-mixed formulation containing both ROX and UDG in an optimized buffer for quality results in real-time quantitative PCR assays. The proprietary reaction buffer with optimum MgCl2 concentration is specifically designed for robust probe hybridization and efficient cleavage of TaqMan probes. Simply add DNA template, primers, probe(s), and water and the reactions are ready for cycling. The master mix contains a chemically-modified Taq DNA Polymerase, MgCl2, ultrapure nucleotides with an optimized dUTP:dTTP ratio, Uracil-DNA Glycosylase (UDG or UNG), and ROX Passive Reference Dye in a proprietary reaction buffer.

Features:
  • One-tube master mix with ROX Reference Dye included
  • Similar protocols, same probes, and same primers for standard mode amplification
  • Consistency over a broad dynamic range: 9 orders of magnitude linear detection range with a correlation coefficient = 0.999
  • Exceptional performance with challenging GC-rich regions
  • Detect 2 copies of target from genomic DNA and differentiate less than a 2-fold differences
  • Contains dUTP and Uracil-DNA Glycosylase (UDG or UNG) for optional carryover contamination prevention.
  • Hot start PCR for room temperature reaction setup
  • Stable at room temperature for 72 hours in a preassembled reaction
  • Validated for use with a variety of 5’ reporter fluorophores and 3’ quenchers, as well as TaqMan MGB containing probes



Simplify the process with a single tube master mix containing UDG and ROX passive reference dye

Catalogue No. Description Unit Size
75650_40_RXN-AFM
VeriQuest Probe qPCR Master Mix (2X)
1 ml for 40 reactions (50 µl)
75650 200 RXN-AFM
VeriQuest Probe qPCR Master Mix (2X)
5 ml for 200 reactions (50 µl)
75650 400 RXN-AFM
VeriQuest Probe qPCR Master Mix (2X)
2 x 5 ml for 400 reactions (50 µl)
75650 1000 RXN-AFM
VeriQuest Probe qPCR Master Mix (2X)
5 x 5 ml for 1,000 reactions (50 µl)
75650 2000 RXN-AFM
VeriQuest Probe qPCR Master Mix (2X)
10 x 5 ml for 2,000 reactions (50 µl)

Literature




Where can I find more information about Affymetrix USB?

Visit the manufacturer page at www.stratech.co.uk/affymetrix, email info@stratech.co.uk or call +44 (0) 1638 782600.
 
Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.

Tuesday, April 1, 2014

Product Focus: VeriKine-HS™ Mouse Interferon Beta Serum ELISA Kit

The versatility of mouse models lends their use in a number of disease and autoimmunity studies. Interferon β plays a pivotal role in the protective response to many infections and diseases due to its antiviral and immune-modulatory activities, and is an important early product of TLR/RLR stimulation.

PBL’s 96-well ELISA kit will equip you with the most sensitive and consistent tool for accurately measuring Mouse IFN-β in serum and plasma matrices. Purified, recombinant mouse IFN-β expressed in mammalian cells is provided as the standard. The sensitivity provided by this kit, to less than 1 pg/ml in serum and plasma, will propel your mouse studies and help uncover insights into the pathology of diseases.

Key Benefits & Features
  • The world’s most sensitive Mouse IFN Beta ELISA
  • Quantifies low level mouse IFN-β in serum, plasma and tissue culture media with an assay range of 0.94-60 pg/ml
  • Reliable serum and plasma spike recoveries of >90%
  • Provides reproducible results with inter- & intra-assay CVs of <10%
  • Total assay time of ~2 hours 


 

Related technical information




Catalogue No. Description Pack Size
42410-1 VeriKine-HS™ Mouse Interferon Beta Serum ELISA Kit
  • Compatibility:Serum, Plasma, Tissue Culture Media
  • Assay Range:0.94-60 pg/ml
1 x 96-well plate
4210-2 VeriKine-HS™ Mouse Interferon Beta Serum ELISA Kit
  • Compatibility:Serum, Plasma, Tissue Culture Media
  • Assay Range:0.94-60 pg/ml
5 x 96-well plate

 

Product Specific Information



 

References

  1. Staehelin, T., Stahli, C., Hobbs, D.S., and Peskta, S. (1981) “A Rapid Quantitative Assay of High Sensitivity for Human Leukocyte Interferon with Monoclonal Antibodies,” Methods in Enzymology, Vol. 79 (S. Peskta, ed.), Academic Press, New York, 589-595.
  2. Balachandran, S,, Thomas, E., and Barber, G. (2004) “A FADD-dependent innate immune mechanism in mammalian cells,” Nature, Vol 432, 401-405.



Where can I find more information about PBL Assay Science?

Visit the manufacturer page at www.stratech.co.uk/pbl_assay_science, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.